منابع مشابه
Eukaryotic transcription initiation
Transcription is defined as the synthesis of RNA from a DNA template. In bacteria, transcription of all genes is catalysed by a single RNA polymerase, whereas in the eukaryotic nucleus, three different RNA polymerases — Pol I, Pol II and Pol III — carry out the synthesis of different classes of RNA. Pol I and Pol III transcribe a limited number of genes encoding ribosomal RNAs, transfer RNAs an...
متن کاملRegulation of the initiation of eukaryotic transcription.
DNA sequences that determine transcriptional regulation of a typical eukaryotic gene consist of a core promoter, which serves as a binding site for the GTF TFIID, and regulatory promoter or enhancer sequences, which bind transcriptional activators. The RNA polymerase II transcription machinery consists of over 50 proteins which are thought to bind to the core promoter in as few as two steps: bi...
متن کاملA common topology for bacterial and eukaryotic transcription initiation?
DNA supercoiling is a major regulator of transcription in bacteria. Negative supercoiling acts both by promoting the formation of nucleoprotein structures containing wrapped DNA and by altering the twist of DNA. The latter affects the initiation of transcription by RNA polymerase as well as recombination processes. Here, we argue that although bacteria and eukaryotes differ in their mode of pac...
متن کاملEukaryotic Initiation Complex Formation
Two distinct pathways have been elucidated which lead to the formation of an AUG-dependent initiation complex. One pathway involves the use of initiation factor Ml (IF-Ml) to promote AUG-dependent binding of the initiator tRNA to the 40 S subunit, followed by joining of the 60 S subunit in the presence of IF-MBA, IF-MBB, and GTP. The second pathway involves the IF-MP-directed binding of initiat...
متن کاملtranscription initiation
Ve showed earlier [101 that the region of the bla promoter of Tn3 protected by the RHA-polymerase (REAP), has the normal size (about 60bp) at RJAP/promoter molar ratio r(2, but rises to about twice this extent as r increases. Ve confirm here that the species corresponding to normal and extended footprint distinguish by their electrophoretic mobilities. Furtheraore, inspection of the complexes b...
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ژورنال
عنوان ژورنال: Current Biology
سال: 2009
ISSN: 0960-9822
DOI: 10.1016/j.cub.2008.11.052